Eight rheumatic diseases with one blood draw

Information provided by Quest Diagnostics

ANA IFA Cascade with IdentRA®* panel combines sensitivity and specificity of various markers to aid in differential diagnosis for the 8 most common rheumatic diseases:

  • Systemic Lupus Erythematosus (SLE)
  • Mixed Connective Tissue Disease
  • Systemic Sclerosis
  • Rheumatoid Arthritis (RA)
  • Sjögren’s Syndrome
  • Polymyositis
  • CREST Syndrome
  • Neuropsychiatric SLE

ANA IFA Cascade with IdentRA®:

  • Screens antinuclear autoantibodies (ANA) using the gold standard, highly sensitive immunofluorescence assay (IFA) with HEp-2 cells
  • Automatically reflexes a positive ANA screen to a tiered cascade of specific antibodies for a complete result
  • IdentRA® panel components include:
    • Widely used RA markers RF (rheumatoid factor) and CCP (cyclic citrullinated peptide)
    • Serum 14-3-3η (eta) protein, a marker for RA that improves identification of early and established RA,1,2,3 in addition to providing prognostic information4,5

 * ANA Screen, IFA, Reflex Titer/Pattern, Reflex Mplx 11 Ab Cascade with IdentRA (test code 94954)


1. Jansen AL, van der Horst-Bruinsma I, van Schaardenburg D, et al. Rheumatoid factor and antibodies to cyclic citrullinated peptide differentiate rheumatoid arthritis from undifferentiated polyarthritis in patients with early arthritis. J Rheumatol. 2002;29:2074-2076.

2. Maksymowych WP, Naides SJ, Bykerk V, et al. Serum 14-3-3η is a novel marker that complements current serological measurements to enhance detection of patients with rheumatoid arthritis. J Rheumatol. 2014;41:2104-2113.

3. Naides SJ, Marotta A. 14-3-3η in “seronegative” rheumatoid arthritis. J Rheumatol. 2015;42(10):1995.

4. Carrier N, Marotta A, de Brum-Fernandes AJ, et al. Serum levels of 14-3-3η protein supplement C-reactive protein and rheumatoid arthritis-associated antibodies to predict clinical and radiographic outcomes in a prospective cohort of patients with recent-onset inflammatory polyarthritis. Arthritis Res Ther. 2016;18:37.

5. van Beers-Tas MH, Marotta A, Boers M, et al. A prospective cohort study of 14-3-3η in ACPA and/or RF-positive patients with arthralgia. Arthritis Res Ther. 2016;18:76.


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