POEMs
Patient-Oriented Evidence That Matters

One Dose of HPV Vaccine Is Noninferior to Two Doses for Preventing HPV Infection

Mark H. Ebell, MD, MS,
AFP Deputy Editor for Evidence-Based Medicine; Professor, Michigan State University, East Lansing

American Family Physician. 2026;114(3):313A-313B.

Author Disclosure: No relevant financial relationships.

CLINICAL QUESTION

Is one dose of human papillomavirus (HPV) vaccine as effective as two doses?

BOTTOM LINE

The large, well-designed study convincingly demonstrates that one dose of HPV vaccine is noninferior to two doses. (Level of Evidence = 1b)

SYNOPSIS

HPV vaccination is highly effective at preventing infection and cervical cancer, but it is underused. Barriers to vaccination are cost and the inability to return for follow-up to receive a second vaccination. The study enrolled unvaccinated females aged 12 to 16 years in Costa Rica from 2017 to 2020, when vaccination in that age group was not routine. Patients with previous infection were excluded. At enrollment, 20,330 participants were randomized to receive a bivalent (subtype 16 and 18 only) or nonavalent vaccine; 6 months later, they were randomized to receive a second dose of the same vaccine or vaccination with a diphtheria, pertussis, and tetanus vaccine as a control to maintain masking. Another group of unvaccinated females aged 16 to 21 years had HPV testing at baseline and at 6 months to allow for determination of vaccine efficacy.

For noninferiority assessment of one dose vs two doses of each vaccine, a noninferiority margin of no more than 1.25 additional infections per 100 women was prespecified. Vaccinated participants were followed up for 5 years with a clinic visit and testing for HPV at each visit from age 15 years onward. Visits occurred annually until age 15 years and then every 6 months. The primary outcome was an HPV infection that lasted at least 6 months and was assessed in the per-protocol population that received two doses (approximately 85% of participants in each group).

Overall vaccine effectiveness compared with the unvaccinated cohort was between 97.0% and 98.5% for all four groups. For the outcome of infection with subtypes 16 or 18, one dose was noninferior to two doses for the bivalent and nonavalent vaccines, with risk differences of −0.13 infections for the bivalent vaccine and +0.22 infections for the nonavalent vaccine per 100 people. For prevention of the nine subtypes in the nonavalent vaccine, one dose was noninferior to two doses (risk difference was +0.56 infections per 100 persons).

Study Design: Randomized controlled trial (double-blinded)

Funding Source: Government and foundation

Allocation: Concealed

Setting: Population-based

Mark H. Ebell, MD, MS, AFP Deputy Editor for Evidence-Based Medicine; Professor, Michigan State University, East Lansing

Author Disclosure: No relevant financial relationships.

  1. 1.Kreimer AR, Porras C, Liu D, et al. Noninferiority of one HPV vaccine dose to two doses. N Engl J Med. 2025;393(24):2421-2433.

POEMs (patient-oriented evidence that matters) are provided by Essential Evidence Plus, a point-of-care clinical decision support system published by Wiley-Blackwell. For more information, see http://www.essentialevidenceplus.com. Copyright Wiley-Blackwell. Used with permission.

For definitions of levels of evidence used in POEMs, see https://www.essentialevidenceplus.com/Home/Loe.

Primary Care Update, a free podcast focused on POEMs, is available on Apple Podcasts and Spotify.

This series is coordinated by Natasha J. Pyzocha, DO, contributing editor.

A collection of POEMs published in AFP is available at https://www.aafp.org/afp/poems.

Copyright © 2026 by the American Academy of Family Physicians.

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